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We followed an excellent genotype of M. lucida once the parent product and established a mini-cut orchard through grafting to provide trunk shoots explants on the lasting. Optimal sterilization was attained using a mixture of 75% ethanol for 30 s, one % benzalkonium bromide for five minutes, and 0.1% mercuric chloride for five minutes. Modified Murashige and Skoog method (ML) had been the suitable medium when it comes to growth of M. lucida. Inclusion of 1 mg/L of 6-benzyl adenine (BA) and 0.05 mg/L of α-naphthaleneacetic