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Despite a globally expanded ensemble, as based on small-angle X-ray scattering, sequence-specific method- and long-range interactions in the cold-unfolded state bring about deviations from homopolymer-like behavior. Our outcomes expose that the cold-denatured state is heterogeneous with regional and long-range intramolecular communications which could prime the folded state also demonstrate that considerable long-range communications tend to be compatible wi