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In our sample of middle-aged and older adults, [Formula see text] was the most robust method to assess [Formula see text]max when indirect calorimetry is available. Although more research is warranted, when indirect calorimetry is not available, a HR of ≤ 4bpm may be a useful alternative to get an accurate representation of maximal effort in middle-aged and older adults. These results suggest that a verification bout is reliable for confirming [Formula see text]max in older adults on both modalities. In our sample of middle-aged and older