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Spectroscopy, together with infrared (IR), Raman, 2D-fluorescence, and total reflection X-ray fluorescence combined with multivariate knowledge evaluation, are often thought-about. Protein Quantification - Quantitation of the full quantity of protein can be accomplished by plenty of assays. Depending on the method, residual oil increases ME but lowers the crude protein (CP) content and this leads to a short shelf life. Prime quality coupling reagents and additives would assist the coupling price and suppress the racemization of amino acid residue