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This in vitro study investigated whether aging of restorative materials influences secondary caries development. The research question was whether antibacterial bonding maintained its effectiveness after aging. 60 enamel-dentine blocks were restored using 3 different restorative materials (n = 20 per material) composite with conventional bonding, composite with antibacterial bonding and amalgam. Half of the samples in each group (n = 1 were artificially aged through exposure to a sucrose biofilm model. Lesion depth was subsequently mea