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Our findings suggested that CLIC4 could be involved in FxOH-induced apoptosis in human CRC.With the advent of the big data era, machine learning methods have evolved and proliferated. This study focused on penalized regression, a procedure that builds interpretive prediction models among machine learning methods. In particular, penalized regression coupled with large-scale data can explore hundreds or thousands of variables in one statistical model without convergence problems and identify yet uninvestigated important predictors. As one