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5% TiO2 and 1.5% ZrO2 groups. The optimal filler concentrations to enhance the flexural strength of PMMA resin were between 3-5% ZrO2, 1.5% TiO2, and 3-7% E-glass fibre. Furthermore, for all composites, a filler concentration of 3 wt.% and above would significantly improve hardness.Increasing numbers of studies seek to characterize the different cellular sub-populations present in mammalian tissues. The techniques "Isolation of Nuclei Tagged in Specific Cell Types" (INTACT) or "Fluorescence-Activated Nuclei Sorting" (FANS) are frequent