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The development of signal amplification systems has attracted much attention and presents a highly challenging objective. Herein, we reveal the amplification processes using a newly synthesized bisthiourea-binaphthyl-polythiophene conjugate. The spectral data, behavior of supramolecular complexation, and thermodynamic parameters with calculation support comprehensively elucidated the factors that control the outcomes of the signal amplification. The present work provides a new perspective on functional chemosensors and an attractive alter