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The ECL biosensor achieved a low detection limit of 4.92 fM within a wide linear range from 10 fM to 100 nM. This approach offers a new insight for ECL efficiency increase and location-controllable strategies with improved reaction efficiency, demonstrating potential in diagnostic analysis.Intermittent subcutaneous (S.C.) injection of teriparatide [PTH (1-34)] is one of the effective therapies to cure osteoporosis. However, a long-term repeated administration of teriparatide by S.C. to the patients is highly challenging. Herein, a triple