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Novel diversity may be mined from databases and de novo sequencing, but functional characterization remains a limiting step to identifying new alleles. Classical breeding approaches augmented by marker-assisted selection offer a means to rapidly assess the function of new variation in coding or regulatory regions to modulate traits. We used the Cyc-B gene ( of tomato (Solanum lycopersicum L.) for a proof of concept because of its role in the production of β-carotene, a provitamin A carotenoid with importance to human nutrition. We mea
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