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Disinfection by-products (DBPs) formed upon water treatment is an emerging issue worldwide. While monitoring of DBP precursors can easily be achieved for high specific UV absorbance (SUVA) organic (6 L/mg·m), low and extremely low SUVA precursors (100 kDa) and produced a strong protein-like fluorescence signal. When SUVA values of O-rich precursors were extremely low ( less then 1 L/mg·m) they were accompanyied by short-wavelength humic-like fluorescence. During DBP tests, high SUVA produced only high yields of carbonaceous DBPs (e.g