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Objective To investigate the protective role of ulinastatin (UTI) on myocardial ischemia-reperfusion (I/R) injury in rats via endoplasmic reticulum stress (ERS)-induced apoptosis pathway. Materials and methods A total of 60 rats were randomly divided into normal group (n=2, myocardial I/R model group (model group, n=2, and myocardial I/R model+UTI treatment group (treatment group, n=2. The myocardial function indicators [creatinine (Scr) and creatine kinase (CK)] were detected. Enzyme-linked immunosorbent assay (ELISA) was perform