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Univariable analysis showed that GWG that exceeded the IOM recommendation might be associated with risk of GDM (PP95 versus P5 -P95 1.45 [1.16-1.81]). CONCLUSIONS Excessive GWG in the first and second trimester might be a risk factor for GDM, which highlights the importance of appropriate weight gain during pregnancy. This article is protected by copyright. All rights reserved.Glucose dehydrogenase (GDH) is a general tool for driving nicotinamide (NAD(P)H) regeneration in synthetic biochemistry. An increasing number of synthetic bioreactions are b