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© 2020 Cognitive Science Society, Inc.Neonatal infectious diseases are a serious threat to the health of newborns. The aim was to establish a new detection method for the simultaneous measurement of (1,3)-β-D-glucan and procalcitonin in serum for the early screening and efficacy testing of neonatal infectious diseases. We established a sandwich dual-label time-resolved fluorescence immunoassay (TRFIA) anti-(1,3)-β-D-glucan/procalcitonin antibodies immobilized on 96-well plates captured (1,3)-β-D-glucan/procalcitonin antigens and then bande