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To compare the performance of 5 synthetic peptide-based ELISAs with that of 3 commercially available immunofluorescent assays (IFAs) for serologic diagnosis of anaplasmosis and ehrlichiosis in dogs. A convenience set of 109 serum samples obtained before and at various times after inoculation for 23 dogs that were experimentally infected with , or and 1 uninfected control dog in previous studies. All serum samples were assessed with 5 synthetic peptide-based ELISAs designed to detect antibodies against , and and 3 whole organism-based I