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To prospectively examine diagnostic error of neuro-ophthalmic conditions and resultant harm at multiple sites. Prospective, cross-sectional study. A total of 496 consecutive adult new patients seen at 3 university-based neuro-ophthalmology clinics in the United States in 2019 to2020. Collected data regarding demographics, prior care, referral diagnosis, final diagnosis, diagnostic testing, treatment, patient disposition, and impact of the neuro-ophthalmologic encounter. For misdiagnosed patients, we identified the cause of error using the
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