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By detecting and assessing the individual ossification areas, therefore simulating the workflow for the Tanner-Whitehouse process, the outcome are interpretable for a radiologist.Herein, a two-photon (TP) ratiometric fluorescent probe (NpFA) was created for detecting formaldehyde (FA) in genuine meals samples, living onion cells and zebrafish by fluorescence resonance energy transfer (FRET) strategy. Especially, a TP fluorophore whilst the donor and a FA turn-on naphthalimid