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Changes in the Ube3a transcripts during postnatal brain development were also evaluated by ddPCR. The ddPCR method is far more reliable and simpler to use than semiquantitative PCR to analyse skewed or faint allelic expression of imprinted genes. Polysaccharide of var. Cheng et L.K.Fu (Taxaceae) (PTM) functions in anti-apoptosis and antioxidation, but its function on Alzheimer's disease (AD) remains unclear. To investigate the effect of PTM on AD. C57BL/6J mice were randomly divided into three groups control, d-galactose (d-gal), and