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To study the prognostic significance of E-cadherin, B-catenin, and Cyclin D1 expression in oral squamous cell carcinoma. The study included 65 subjects with histologically confirmed squamous cell carcinoma. TMA blocks were prepared for immunohistochemical quantification of the expression of the three markers using IHC profiler and Immune ratio plugin of Image J. E-cadherin expression was significantly correlated with histological grades and the metastasis status (p0.05), whereas B-catenin expression was significantly correlated with sm