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To simulate up to 48 hours continuous aerosol publicity during mechanical ventilation, a GLP toxicology research had been performed utilizing anesthetized, intubated, mechanically ventilated dogs. Dogs inhaled epoprostenol at approximately 6× and 13× the dose rate reported in off-label individual scientific studies. This novel animal model required organization of your pet dog intensive treatment product offering sedation, multisystem help, limited parenteral diet, and handling of