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Development of ratiometric imaging techniques to quantitatively measure hypoxia levels in vivo is essential in predicting the effectiveness of cancer treatments and diagnostics. With complementary advantages of near-infrared fluorescent (NIRF) and photoacoustic (PA) imaging, NIRF/PA dual-ratiometric probes are imperative for precisely visualizing tumor hypoxia levels. However, because the absorbed excitation energy is fixed in a single chromophore, its NIRF and PA effects are always competitive, which greatly thwarts the development of