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As dietary magnolol levels increased, villus height of jejunum and ileum linearly increased (P less then 0.01). M200 and M300 groups had higher expression level of occludin in the ileum compared with group C (P less then 0.01). The level of nitric oxide production and inducible nitric oxide synthase expression in the ileum of M200 group were lower than that in the C group (P less then 0.05). In conclusion, dietary supplementation of 200 and 300 mg/kg magnolol can improve hen performance, albumen quality of fresh and storage eggs, and he