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Members had been instructed to get feces samples twice spaced by a week, utilizing six various methods (naive and Zymo DNA/RNA Shield on dry ice, OMNIgene GUT, RNALater, 95% ethanol, Zymo DNA/RNA Shield at room-temperature). DNA extraction from all samples was done relatively using QIAamp Power Fecal and ZymoBIOMICS DNA kits. 16S rRNA sequencing of the gut microbiota along with qPCRs had been performed regarding the remote DNA. Metrics included alpha diversitedures in clinical research. TRANSLATIO
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