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Compounds b2, b11and b19 reversibly inhibited urease with a combined system, and showed exemplary effectiveness against both cell-free urease and urease in undamaged cellular, with IC50 values being 90-to 450-fold and 5-to 50-fold less than the good control acetohydroxamic acid, respectively. Probably the most powerful ingredient b11 revealed IC50 value of 0.060 ±0.004μM against cell-free urease, which bound to urea binding site with an extremely low KDvalue