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We applied the technique in magnetic tweezers and monitored the 3D position of over 100 beads in realtime on a generic CPU. The accuracy of 3D phasor monitoring ended up being extensively tested and compared to a look-up table approach utilizing Lorenz-Mie simulations, avoiding experimental uncertainties. Its easy execution, effectiveness, and robustness can enhance multiplexed biophysical bead-tracking programs, especially when high throughput is necessary and image artifacts ar