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Microbiota plays a prominent role in periodontal disease, but the canine oral microbiota and how dental chews may affect these populations have been poorly studied. We aimed to determine the differences in oral microbiota of adult dogs consuming dental chews compared to control dogs consuming only a diet. Twelve adult female beagle dogs (mean age = 5.31 ± 1.08 yr) were used in a replicated 4×4 Latin square design consisting of 28-d periods. Treatments (n=12/group) included Diet only (CT); Diet + Bones Chews Dental Treats (BC; Chewy
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