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Therefore, our conclusions prove that the liposome distribution system reveals more effectiveness to adjuvant TB subunit vaccine than O/W emulsion and emphasize the importance of adjuvant formula for the better effectiveness of a protein vaccine.KCNQ1OT1 exerts an important role in various types of cancer, but its role in osteosarcoma (OS) therefore the potential apparatus remain to be clarified. In our research, we aimed to explore the effect of KCNQ1OT1 on