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We propose a novel and simple method for measuring vertical distances in the SEM, which is useful for in-situ measurements and nanomanipulations.The main objective of this study was to analyze the changes in osteoblast behaviors by two different methods, a simple digital image analysis method and conventional assays. Osteoblast progenitor cells and osteoblasts were treated with alendronate (ALN; a nitrogen-containing bisphosphonate). Osteoblast behaviors such as the uptake of ALN, cell proliferation, cell differentiation, and mineralized
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