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This study could serve as the starting point for further investigation of these molecules through in vitro and in vivo assays. Toxic pollutants leaching from littered cigarette butts (C raise environmental impact concerns. The US Food and Drug Administration (FDA) is required to assess the environmental impacts of its tobacco regulatory actions per the US National Environmental Policy Act (NEPA). We determined the chemical constituents in CB leachate through analyses of 109 field-collected CB and literature compilation and characterized