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truncatula require DREPP in order to regulate MT reorganization during initial root hair responses to rhizobia. © 2020 American Society of Plant Biologists. All rights reserved.Maize is one of the most important crops in the world. However, few agronomically important maize genes have been cloned and used for trait improvement, due to the complex genome and genetic architecture. Here we integrated multiplexed CRISPR/Cas9-based high-throughput targeted mutagenesis with genetic mapping and genomic approaches to successfully knock-out 743