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CLINICAL SIGNIFICANCE It may be prudent to rest any cat that has had a TPU or extended TPU to reduce fracture risk. © 2020 The American College of Veterinary Surgeons.Nature provides a large number of functional proteins that evolved during billions of years of evolution. The diversity of natural proteins encompasses versatile functions and more than a thousand different folds, which, however, represents only a tiny fraction of all possible folds and polypeptide sequences. Recent advances in the rational design of protein