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SND2 appears to function downstream to amplify NARS1 via positive feedback. This novel regulatory mechanism expands our understanding of the sophisticated vascular tissue patterning processes occurring during the postembryonic root development. © 2020 American Society of Plant Biologists. All rights reserved.Calcium-regulated protein kinases are key components of are key components of intracellular signaling in plants that mediate rapid stress-induced responses to changes in the environment. To identify in vivo phosphorylation substrate