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Here, we detail available methods for TGF-β biological activity analysis, together with their applicability and suitability for each experimental setting, in order to get a complete analytical perspective and more comprehensive information along the development and design of combined antitumor immunotherapies, which include the inhibition of TGF-β biological activity. © 2020 Elsevier Inc. All rights reserved.The past two decades witnessed the appreciation of the importance of specific tumor-infiltrating immune ce