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The recommended strategy was placed on separation prediction of BPs with a high accuracy. Beneath the enhanced conditions, good linearity had been obtained utilizing the correlation coefficients (R2) which range from 0.998 to 1.000. The recoveries in spiked examples were 91.3-110.7% with the intra-day and inter-day relative standard deviation ranging 0.4-9.6% and 0.5-10.2%, correspondingly. The limitations of recognition and quantification were 0.13-66.7 ng L-1 and 0.40-200 ng L-1. The evolve