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A two-stage bioreactor operated under anoxic denitrifying conditions was evaluated for desulfurization of synthetic biogas laden with H2S concentrations between 2500 and 10,000 ppmv. H2S removal efficiencies higher than 95% were achieved for H2S loads ranging from 16.2 to 51.9 gS mliquid-3h-1. Average H2S oxidation performance (fraction of S-SO42- produced per gram of S-H2S absorbed) ranged between 8.2 ± 1.2 and 18.7 ± 5.3% under continuous liquid operation. Nitrogen mass balance showed that only 2-6% of the N-NO3- consumed was directe