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Growth of generalist herbivores from two feeding guilds, Myzus persicae Sulzer (Hemiptera Aphididae; green peach aphid) and Trichoplusia ni Hübner (Lepidoptera Noctuidae; cabbage looper), was decreased on the mutant line compared to wildtype. Both herbivores accumulated cardenolides in proportion to the plant content, with T. ni accumulating higher total concentrations than M. persicae. Helveticoside, a relatively abundant cardenolide in E. cheiranthoides, was not detected in M. persicae feeding on these plants. Our results support the