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Both postgraduate residents and nurses showed moderately satisfactory knowledge, attitude and practice towards hand hygiene. To assess knowledge and practices regarding hand-washing among mothers of children aged ≤5 years. The cross-sectional study was conducted from March to July 2015 in rural areas of Union Council Charwa, Tehsil Pasrur in District Sialkot, Pakistan, and comprised mothers of children aged ≤5 years. Data was collected using a pre-tested and validated structured questionnaire developed in line with World health Organisa