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23 meV energy and 66.12% at 1.10 meV energy. The secondary gamma rays were found to increase by 94.17% at 0.23 meV energy and 63.74% at 1.10 meV energy. The encapsulation of the source attenuated the gamma rays, which altered the spectrum. The mean energy of the beam increased, thereby exhibiting a beam-hardening effect. Currently, data on pathogenic variants in the CHEK2 gene and their impact on cancer risk are lacking. This study aimed to explore the characteristics of breast cancer (BC) patients from families with CHEK2 pathogenic vari