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Objective To evaluate the contribution of suprahyoid muscles to mouth opening in different hyoid bone positions.Methods The jaw-opening and closing movements were imposed on the 3D inverse dynamic jaw model with and without the lateral pterygoid muscle (LPM). The activation of the muscles was evaluated for eight different positions of the hyoid bone.Results The muscles with 100% activation provided maximum mouth opening (MMO). When the hyoid bone was replaced, the muscles could achieve MMO at the 135º, 180º, and 225º points with the L