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SIGNIFICANCE Extracellular vesicles (EVs) are important both at normal physiological processes as well as pathological progression during pathogen and host interaction. In this paper we first establish the extraction method of EVs from the important oomycete pathogen Phytophthora capsici. Bioinformatics analysis of EV proteomics revealed a variety of pathogenic-related proteins, like oxidation/reduction-related proteins, stress response proteins as well as elicitors. Our results will help better understanding the biological function of