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05) higher expression levels of CLDN3 (26x), CLDN15 (29x), OCLN (4x), and TJP1/ZO-1 (5x) than invitro HIOs. There was no significant difference in expression of these tight junction components between 4- and 8-week-old tHIOs. Immunofluorescent staining revealed the presence of claudin 3, claudin 15, occludin, and zonula occludens-1 in both invitro HIOs and tHIOs; however, the morphology appeared more mature in tHIOs. Invitro HIOs have lower levels of tight junction mRNA, and tight junction proteins appear morphologically immature. Tran
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