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SA-mediated formation of proline and flavonoids was linked with the enhanced activity of Δ1-pyrroline-5-carboxylate synthetase and phenylalanine ammonia lyase. Fluoride stress enhanced the activity of enzymes like glyoxalase I and glyoxalase II which were further aggravated in the seedlings upon treatment with SA, effectively detoxifying the methylglyoxal formed during stress. Overall, the manuscript depicts the pivotal role played by exogenous SA in ameliorating the effects of fluoride-induced damages in the seedlings and proves its potentiality as