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But, whenever meals by-products and biomass are used as feedstocks, impurities often decrease output. Using metabolic profiling, sugar had been defined as a potential inhibitor of ammonia manufacturing from impure food by-products. We built the recombinant Escherichia coli, for which sugar uptake had been paid down by ptsG gene disturbance and amino acid catabolism had been promoted by glnA gene interruption. Ammonia production effectiveness from okara, a food by-pro