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The enveloped flavivirus Israel turkey meningoencephalitis virus (ITV) causes a neuroparalytic disease in adult turkeys leading to morbidity and mortality. This study reevaluates the role of blood-sucking insects in the transmission of ITV. We demonstrate the crucial importance of two factors in detecting viruses carried by blood-sucking insects first, enhanced molecular detection of ITV in insects by nested qRT-PCR and second, collection and maintenance of live insects until their molecular examination. These upgrades allowed overcomin
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