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Therefore, a simple semicontinuous incubation system has been developed, which studies the rate and extent of fermentation by gas production at the time it allows for controlling medium pH and rate of passage by manual replacement of incubation medium by fresh saliva without including rumen inoculum. The application of this system to studies using high concentrate feeding conditions will also be reviewed here.Various studies have indicated that particulate matter less then 2.5 μm (PM2.5) could cause adverse health effects on pulmonary functions in s