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The formation of β1,3-linkages on animal glycoconjugates is catalyzed by a subset of β1,3-glycosyltransferases grouped in the CAZy family GT31. This family represents an extremely diverse set of β1,3-N-acetylglucosaminyltransferases (B3GNTs and FNGs), β1,3-N-acetylgalactosaminyltransferases (B3GALNTs), β1,3-galactosyltransferases (B3GALTs and C1GALTs), β1,3-glucosaminyltransferase (B3GLCT), and β1,3-glucuronyl acid transferases (B3GLCATs or CHs). The mammalian enzymes were particularly well-studied and shown to use a large variety of sug