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The regeneration associated with the pulp-dentin complex regeneration in certain, features drawn the eye of several researchers due to the high medical requirements. While it is still essential to execute in vitro study making use of numerous cells, scaffolds and growth facets, it is also crucial to possess a dependable pet design for preclinical tests. In this chapter, we explain a mouse model in which a scaffold resembling a tooth containing dental pulp cells is implanted subcutaneously.