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1% (95% CI 53.6% - 70.2%), taking trace outcomes as positive according to the manufacturer's instructions. Retesting urine from the 140 HTX-negatives after one-year storage at -20 °C with two new POCCCA batches simultaneously yielded significantly different specificities (34.3%; 95%CI 26.5% - 42.8% and 75.0%; 95% CI 67.0% - 81.9%). These two batches had a weak agreement (Cohen's kappa 0.56; 95%CI 0.44-0.68) among the 174 urine samples retested. At present, POCCCA cannot be recommended either as a cut-off point for MDA or a relia