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After the enzymatic saccharification, 2,3-butanediol (2,3-BD) fermentation ended up being done utilizing an engineered S. cerevisiae strain. The application of HT acid-hydrolyzed method with 1.9 g/l of 5-hydroxymethylfurfural demonstrated a reduction in the lag time from 48 to 24 h. The 2,3-BD concentration and yield coefficient at 72 h had been 14.8 g/l and 0.30, respectively. Therefore, HT acid hydrolysis plus the utilization of the designed S. cerevisiae strain can boost the entir
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