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But, the formation of [M+NH4]+ adducts of TGs is less efficient compared to development of [M+K]+ adducts into the number of AF concentrations from 0.1 to 10 mM. Chemical derivatization using 100 μM of Girard T reagent predominately generates reaction services and products of phosphatidylcholine rather than TG types. Additionally, the current presence of the Girard T reagent suppresses ion signals of all types into the spectrum in